Selected project
Hmgn3
Graphics
Graphic caption: Flow Cytometry: FACS pie chart, split by sex and genotype
Graphic caption: Immunglobulins: Concentration in blood plasma of female mice, boxplot, split by genotype
Graphic caption: Immunglobulins: Concentration in blood plasma of male mice, boxplot, split by genotype
Graphic caption: Total IgE in Plasma: IgE boxplot with stripchart, split by sex and genotype
Tables
Table caption: Flow Cytometry: Frequencies of Leukocyte subpopulations in peripheral blood after erythrocyte lysis [percentage of all leukocytes (CD45+ cells), or corresponding parent gate, respectively]. Means, standard deviation and p-values calculated by a linear model
femalemaleLinear modelLinear modelLinear model
 controlmutantcontrolmutantgenotypesexgenotype:sex
 n=10n=10n=10n=10   
 mean ± sdmean ± sdmean ± sdmean ± sdp-valuep-valuep-value
CD45+/T cells 49.11 ± 2.9550.17 ± 6.5942.12 ± 2.443.27 ± 2.110.382< 0.0010.971
CD45+/CD3+CD4+ 20.15 ± 2.5720.85 ± 3.5118.92 ± 1.6319.95 ± 1.150.260.170.827
CD45+/CD3+CD8+ 24.6 ± 1.3625.75 ± 3.2518.57 ± 2.4119.55 ± 2.090.164< 0.0010.909
CD45+/B cells 10.21 ± 1.549.24 ± 1.6713.17 ± 2.3913.43 ± 2.340.586< 0.0010.345
CD45+/CD5-NK+ 8.54 ± 2.168.71 ± 2.056.97 ± 0.876.34 ± 1.60.6860.0010.469
CD45+/CD11b+Gr1+ 18.86 ± 2.3118.71 ± 4.4324.53 ± 3.3423.74 ± 3.330.668< 0.0010.77
CD45+/NK-Gr1-CD11b+ 4.81 ± 0.94.59 ± 0.856.6 ± 1.126.39 ± 1.230.516< 0.0010.988
CD3+CD4+/CD25+ 6.68 ± 1.145.72 ± 1.048.97 ± 0.718.66 ± 1.060.054< 0.0010.31
CD45+/CD3+gammadeltaTCR+ 0.52 ± 0.120.56 ± 0.110.29 ± 0.090.28 ± 0.050.49< 0.0010.391
CD3+CD4+/CD62L+ 23.83 ± 10.1819.32 ± 10.8449.53 ± 14.3452.09 ± 9.660.789< 0.0010.334
CD3+CD4+/CD44+ 67.68 ± 2.6568.18 ± 1.9965.59 ± 3.0465.36 ± 2.570.870.0050.658
CD3+CD8+/CD62L+ 35.18 ± 10.7332.52 ± 13.8861.13 ± 15.9965.74 ± 8.990.809< 0.0010.371
CD3+CD8+/CD44+ 56.66 ± 2.3255.82 ± 1.9956 ± 2.7355.78 ± 2.350.4820.6420.681
CD45+/CD5+NK+ 0.95 ± 0.110.97 ± 0.10.76 ± 0.140.72 ± 0.090.695< 0.0010.317
B cells/IgD+ 57.03 ± 5.2759.46 ± 6.4271.81 ± 2.9771.45 ± 3.770.499< 0.0010.364
B cells/CD5+ 5.97 ± 0.797.1 ± 2.794.38 ± 0.834.35 ± 0.790.269< 0.0010.245
B cells/B220+MHCclassII+ 55.55 ± 4.0157.29 ± 4.5466.37 ± 3.2966.34 ± 2.720.47< 0.0010.455
NK+/CD11b+ 74.86 ± 3.6874.33 ± 2.6460.49 ± 4.5556.45 ± 4.290.069< 0.0010.159
CD3+ rest 4.36 ± 0.943.57 ± 0.844.62 ± 1.633.76 ± 1.460.0450.5720.93
CD45+/CD3+CD4+CD25+ 1.35 ± 0.291.19 ± 0.311.69 ± 0.161.73 ± 0.250.472< 0.0010.243
ratio CD4:CD8 0.82 ± 0.10.81 ± 0.061.03 ± 0.111.03 ± 0.120.877< 0.0010.829
ratio Granulocytes:(Tcells+B cells) 0.32 ± 0.050.32 ± 0.10.45 ± 0.080.42 ± 0.080.698< 0.0010.587
ratio Tcells:Bcells 4.91 ± 0.755.63 ± 1.433.29 ± 0.63.3 ± 0.550.205< 0.0010.221
Table caption: Flow Cytometry: Frequencies of Leukocyte subpopulations in peripheral blood after erythrocyte lysis [percentage of all leukocytes (CD45+ cells), or corresponding parent gate, respectively]. Medians, first and third quartile and p-values calculated by a Wilcoxon rank-sum test$\^{a}$ Number not based on the full number of animals (missing values)
femalemalefemalemaleoverall
 controlmutantcontrolmutant   
 n=10n=10n=10n=10   
 median
[25%, 75%]
median
[25%, 75%]
median
[25%, 75%]
median
[25%, 75%]
p-valuep-valuep-value
CD45+/T cells 49.45
[47.8 , 51.5]
48.75
[46.5 , 54.65]
41.1
[40.42 , 43.9]
43.2
[41.92 , 44.55]
0.9560.2390.784
CD45+/CD3+CD4+ 20
[18.59 , 22.21]
20.22
[19.12 , 22.46]
18.66
[17.97 , 19.45]
19.95
[18.9 , 21.07]
0.6310.1650.245
CD45+/CD3+CD8+ 24.42
[23.72 , 25.51]
25.24
[23.2 , 28.02]
18.28
[16.68 , 20.75]
19.43
[17.93 , 21.4]
0.5290.280.565
CD45+/B cells 10.11
[9.27 , 11.3]
8.7
[8.07 , 10.31]
12
[11.68 , 14.55]
12.6
[11.82 , 14.3]
0.1710.8530.673
CD45+/CD5-NK+ 8.25
[6.98 , 9.99]
8.8
[7.38 , 9.75]
6.7
[6.28 , 7.43]
6.89
[5.51 , 7.28]
0.9710.4360.841
CD45+/CD11b+Gr1+ 18.55
[17.05 , 20.43]
17.4
[16.3 , 22.27]
24.95
[22.45 , 25.8]
23.85
[22.65 , 25.45]
0.9270.6710.888
CD45+/NK-Gr1-CD11b+ 4.94
[3.91 , 5.62]
4.76
[4.3 , 5.12]
6.46
[5.73 , 6.77]
5.85
[5.46 , 7.13]
0.6310.6310.461
CD3+CD4+/CD25+ 6.46
[5.87 , 7.03]
5.96
[5.35 , 6.21]
9.13
[8.47 , 9.36]
8.73
[8.13 , 9.29]
0.1230.6840.327
CD45+/CD3+gammadeltaTCR+ 0.55
[0.41 , 0.61]
0.54
[0.51 , 0.64]
0.26
[0.24 , 0.35]
0.28
[0.24 , 0.32]
0.36210.753
CD3+CD4+/CD62L+ 21.2
[15.65 , 32.5]
15.85
[12.22 , 28.25]
53.75
[39.23 , 59.52]
55.55
[43.25 , 58.5]
0.2180.7540.805
CD3+CD4+/CD44+ 67.4
[65.83 , 70.08]
67.95
[66.85 , 69.78]
65.9
[62.88 , 67.78]
65.25
[63.9 , 67.05]
0.7390.9860.825
CD3+CD8+/CD62L+ 34.95
[30.05 , 42.62]
28.05
[23.35 , 45.62]
66.55
[49.02 , 69.55]
70.45
[56.73 , 73.25]
0.5790.5790.82
CD3+CD8+/CD44+ 57.05
[55.5 , 57.8]
55.85
[54.25 , 56.73]
56.9
[54.27 , 57.5]
55.85
[53.88 , 57.77]
0.3240.9120.441
CD45+/CD5+NK+ 0.96
[0.88 , 1.03]
0.96
[0.92 , 1.03]
0.75
[0.67 , 0.84]
0.71
[0.64 , 0.8]
0.7960.3830.683
B cells/IgD+ 55.55
[53.48 , 59.85]
60.1
[53.77 , 63.08]
71.35
[69.35 , 73.58]
71.35
[69.33 , 72.92]
0.4040.8530.774
B cells/CD5+ 6.2
[5.57 , 6.4]
6.64
[5.69 , 7.29]
4.34
[3.68 , 4.99]
4.39
[3.7 , 4.63]
0.3050.9710.753
B cells/B220+MHCclassII+ 55.3
[54.23 , 56.17]
56.6
[53.48 , 60.15]
66.85
[65.9 , 68.55]
66.8
[64.97 , 68.25]
0.4030.8680.753
NK+/CD11b+ 75.25
[72.83 , 76.3]
74.4
[72.65 , 75.6]
61
[56.08 , 63.17]
57.3
[52.77 , 59.38]
0.7550.1650.534
CD3+ rest 4.28
[3.55 , 5.09]
3.36
[3.2 , 4.15]
4.48
[3.62 , 5.73]
3.41
[2.58 , 4.7]
0.0890.280.048
CD45+/CD3+CD4+CD25+ 1.31
[1.17 , 1.34]
1.1
[1.03 , 1.24]
1.7
[1.6 , 1.83]
1.73
[1.56 , 1.95]
0.0890.5290.583
ratio CD4:CD8 0.84
[0.72 , 0.87]
0.8
[0.78 , 0.83]
1
[0.96 , 1.12]
1
[0.96 , 1.06]
0.6950.9850.804
ratio Granulocytes:(Tcells+B cells) 0.31
[0.28 , 0.35]
0.3
[0.26 , 0.41]
0.44
[0.39 , 0.5]
0.44
[0.39 , 0.46]
10.7251
ratio Tcells:Bcells 4.7
[4.61 , 5.41]
5.95
[4.18 , 6.55]
3.44
[2.83 , 3.79]
3.41
[2.88 , 3.66]
0.3530.9880.683
Table caption: Immunglobulins: Levels of immunoglobulins (microgram per milliliter) in blood plasma. Changes in the IgG1/IgG2b ratio are indicative of changes in the Th1/Th2 balance. Medians, first and third quartile and p-values calculated by a Wilcoxon rank-sum test. Missing measurements caused by Ig levels above and below measurability are replaced by 0.9*min / 1.1*max of respective Ig measurement.$\^{a}$ Number not based on the full number of animals (missing values)
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