Selected project
Cip2a
Graphics
Graphic caption: Flow Cytometry: CD3+CD4+/CD44+ boxplot with stripchart, split by sex and genotype
Graphic caption: Flow Cytometry: CD3+CD4+/CD44+ vs. CD3+CD8+/CD44+ scatterplot
Graphic caption: Flow Cytometry: CD3+CD8+/CD44+ boxplot with stripchart, split by sex and genotype
Graphic caption: Flow Cytometry: FACS pie chart, split by sex and genotype
Graphic caption: Total IgE in Plasma: IgE boxplot with stripchart, split by sex and genotype
Tables
Table caption: Frequencies of Leukocyte subpopulations in peripheral blood after erythrocyte lysis [percentage of all leukocytes (CD45+ cells), or corresponding parent gate, respectively]. Means, standard deviation and p-values calculated by a linear model
femalemaleLinear modelLinear modelLinear model
 controlmutantcontrolmutantgenotypesexgenotype:sex
 n=10n=10n=10n=10   
 mean ± sdmean ± sdmean ± sdmean ± sdp-valuep-valuep-value
CD45+/T cells 23.45 ± 3.5425.49 ± 5.5a19.83 ± 2.5117.53 ± 3.50.917< 0.0010.088
CD45+/CD3+CD4+ 12.19 ± 2.0612.91 ± 2.65a10.39 ± 1.778.42 ± 2.760.409< 0.0010.082
CD45+/CD3+CD8+ 9.97 ± 1.3410.25 ± 2.06a8.7 ± 0.938.21 ± 1.440.8220.0010.422
CD45+/B cells 59.98 ± 3.16a58.12 ± 4.92a64.73 ± 1.8262.89 ± 7.120.2370.0040.996
CD45+/CD5-NK+ 2.12 ± 0.79a1.52 ± 0.59a2.81 ± 0.412.84 ± 0.340.112< 0.0010.088
CD45+/CD11b+Gr1+ 4.57 ± 2.05a5.92 ± 3.76a6.74 ± 1.9310.01 ± 7.730.1310.0440.523
CD45+/NK-Gr1-CD11b+ 3.42 ± 0.64a2.63 ± 0.89a2.79 ± 0.63.34 ± 1.170.6670.8710.023
CD3+CD4+/CD25+ 4.41 ± 0.64.37 ± 0.74a5.69 ± 0.475.88 ± 1.290.782< 0.0010.673
CD45+/CD3+gammadeltaTCR+ 0.42 ± 0.060.47 ± 0.1a0.24 ± 0.030.32 ± 0.10.018< 0.0010.629
CD3+CD4+/CD62L+ 58.86 ± 15.6865.92 ± 10.63a63.51 ± 15.6260.24 ± 16.960.6960.9150.291
CD3+CD4+/CD44+ 67.82 ± 1.972.54 ± 2.93a65.03 ± 3.7269.76 ± 7.320.0020.0610.998
CD3+CD8+/CD62L+ 73.84 ± 13.9279.11 ± 10.7a76.88 ± 14.0475.8 ± 15.340.6360.9760.474
CD3+CD8+/CD44+ 63.4 ± 3.4566.7 ± 2.66a64.38 ± 2.9367.94 ± 9.590.0610.5350.942
CD45+/CD5+NK+ 0.11 ± 0.04a0.12 ± 0.07a0.11 ± 0.030.09 ± 0.040.6080.3430.422
B cells/IgD+ 85.47 ± 2.31a83.96 ± 5.67a92.12 ± 0.8890.57 ± 3.480.187< 0.0010.986
B cells/CD5+ 1.58 ± 0.21a1.71 ± 0.33a1.55 ± 0.41.32 ± 0.530.6860.1120.163
B cells/B220+MHCclassII+ 30.59 ± 13.24a29.99 ± 17.61a61.54 ± 5.3263.6 ± 7.670.849< 0.0010.729
NK+/CD11b+ 79.32 ± 27.9986.58 ± 3.23a81.15 ± 3.5183.84 ± 5.540.2970.9240.63
CD4/CD8 1.22 ± 0.071.27 ± 0.11a1.19 ± 0.131.05 ± 0.330.4370.050.122
Table caption: Frequencies of Leukocyte subpopulations in peripheral blood after erythrocyte lysis [percentage of all leukocytes (CD45+ cells), or corresponding parent gate, respectively]. Medians, first and third quartile and p-values calculated by a Wilcoxon rank-sum test$. \^{a}$ Number not based on the full number of animals (missing values)
femalemalefemalemaleoverall
 controlmutantcontrolmutant   
 n=10n=10n=10n=10   
 median
[25%, 75%]
median
[25%, 75%]
median
[25%, 75%]
median
[25%, 75%]
p-valuep-valuep-value
CD45+/T cells 22.65
[20.93 , 26.7]
26.6a
[20.5 , 28]
20.4
[18.45 , 21.52]
16.7
[15.45 , 20.55]
0.4340.1090.573
CD45+/CD3+CD4+ 12.2
[10.55 , 13.5]
12.8a
[10.2 , 14.3]
10.7
[9.67 , 11.68]
8.66
[7.55 , 10.75]
0.7050.1280.432
CD45+/CD3+CD8+ 9.8
[8.89 , 10.93]
10.6a
[8.48 , 11.4]
8.73
[8.02 , 9.37]
7.92
[7.08 , 8.68]
0.6750.2180.545
CD45+/B cells 59.2a
[58.1 , 59.9]
56.2a
[56 , 57.7]
64.9
[63.92 , 65.83]
65.6
[61.15 , 67.2]
0.0770.6840.511
CD45+/CD5-NK+ 1.88a
[1.62 , 2.62]
1.48a
[1.09 , 2.1]
2.77
[2.6 , 3.02]
2.88
[2.61 , 3]
0.1420.7960.374
CD45+/CD11b+Gr1+ 5.01a
[2.78 , 6.35]
4.16a
[3.54 , 7.37]
6.29
[5.35 , 8.12]
6.54
[5.8 , 9.57]
0.4740.5290.406
CD45+/NK-Gr1-CD11b+ 3.16a
[2.98 , 3.91]
2.83a
[2 , 3.19]
2.8
[2.33 , 3.3]
2.96
[2.58 , 3.69]
0.1360.3420.568
CD3+CD4+/CD25+ 4.29
[3.95 , 5.01]
4.34a
[3.95 , 5.05]
5.76
[5.33 , 6.05]
5.7
[4.94 , 6.9]
0.9050.9120.835
CD45+/CD3+gammadeltaTCR+ 0.41
[0.38 , 0.42]
0.46a
[0.41 , 0.53]
0.24
[0.23 , 0.26]
0.29
[0.24 , 0.32]
0.2320.0740.167
CD3+CD4+/CD62L+ 59.2
[52.8 , 72.67]
69.3a
[59.2 , 74.6]
72.2
[51.75 , 74.9]
53.9
[46.12 , 76.35]
0.4470.9550.786
CD3+CD4+/CD44+ 67.9
[66.83 , 68.92]
71.2a
[70.9 , 74.6]
65.4
[62.52 , 68.4]
70.1
[63.2 , 72.4]
< 0.0010.1010.001
CD3+CD8+/CD62L+ 74.2
[67.38 , 85.85]
80.9a
[75.7 , 87.8]
83.2
[65.62 , 86.08]
81.65
[62.15 , 88.45]
0.40.9710.687
CD3+CD8+/CD44+ 64.15
[61.83 , 66.1]
67a
[65.5 , 68.2]
64.3
[63.52 , 66.62]
66
[61.45 , 67.2]
0.0260.7390.06
CD45+/CD5+NK+ 0.1a
[0.09 , 0.14]
0.1a
[0.07 , 0.14]
0.1
[0.09 , 0.12]
0.08
[0.07 , 0.12]
0.8160.2560.292
B cells/IgD+ 85.1a
[83.8 , 86.9]
82.8a
[80.3 , 88.7]
92.3
[91.22 , 92.78]
91.85
[90.03 , 92.97]
0.650.4480.43
B cells/CD5+ 1.63a
[1.45 , 1.74]
1.72a
[1.63 , 1.9]
1.49
[1.31 , 1.88]
1.24
[0.98 , 1.53]
0.2860.3050.745
B cells/B220+MHCclassII+ 24.2a
[18 , 43.9]
29.7a
[17.7 , 42.5]
62.4
[59.25 , 65.75]
64.5
[60.17 , 68.62]
0.7130.4040.801
NK+/CD11b+ 88.45
[84.92 , 90.1]
87.4a
[86.4 , 88.2]
80.75
[79.08 , 81.8]
83.2
[79.78 , 88.78]
0.460.2560.612
CD4/CD8 1.23
[1.16 , 1.25]
1.25a
[1.2 , 1.33]
1.19
[1.09 , 1.3]
1.15
[1.05 , 1.27]
0.3660.4680.917
Media files
Media caption: Analysis of Ova-specific CD8+ T lymphocytes from control and mutant mice 5 days after recall infection with high-dose L.m.-Ova and unchallenged control mice.
Media caption: Bacterial burden in spleens of challenged mice.
Media caption: Intracellular cytokine staining of splenocytes from control and mutant mice on day 7 after primary infection.
Media caption: Flow cytometric analysis of peripheral blood (A, B) and splenocytes (C, D) 2 days after recall infection.
Media caption: Bacterial burden in spleen (A) and liver (B) of infected mice on day 3 after primary infection.
Media caption: Cytokine production by antigen-specific T cells from mutant and control mice on day 5 after recall infection with high-dose L.m.-Ova and unchallenged control mice.
Media caption: Flow cytometric analysis of splenocytes 5 days after recall infection.